4 Well Chamber Slide Cell Density

I am using 4 chamber slides for seeding hek 293t and hek 293 and rat hypothema cell lines.

4 well chamber slide cell density. Nunc lab tek chamber slide system 4 wells on glass working volume 0 5 0 9 ml. It allows for growth and staining of cells in one go. Grow stain and examine cells directly on the thermo scientific nunc lab tek ii chamber slide which consists of a removable polystyrene media chamber attached to a standard glass slide. Dilute the cell suspension in cell culture media to a final concentration of 0 5 5 10 5 cells ml 1.

Remove the medium gently. Glass slides are specially cleaned for optimal cell attachment and growth. Count the number of viable cells using a hemacytometer. Cell culture chamber chamber slides microscope slides.

For chamber slides add the recommended volume of cell suspension into each well of the chamber slide depending on the size of the well. A cell culture chamber for observation of the sample through the coverslip like bottom using high resolution microscopy. Biocompatible acrylic adhesive seals the media chamber and slide preventing well to well contamination. Nunc lab tek chamber slide system 4 wells permanox slide 1 8 cm2 well sterile 96 cs.

An open µ slide chambered coverslip with 4 wells for use in immunofluorescence and high end microscopy. A chamber slide consists of a removable media chamber attached to a slide treated for adherent cell culture. I add 30 50k cells in each well of two well labtek chamber. I am using rpmi 1640 from sigma with 10 fbs 1 gutamax and p s.

Find null c6932 msds related peer reviewed papers technical documents similar products more at sigma aldrich. The lab tek ii cc 2 chamber slide features a chemically modified growth surface that mimics the characteristics of poly lysine coating. Separate the media chamber from the slide using removal tool included. Grow the cells in endothelial cell growth medium to 95 confluent.

I am now using the lab tek chamber slide to do the cell biology experiment. Available in 1 2 4 and 8 well formats. All in one chamber slide used for cell culture and microscopy. Plate endothelial cells on the coated chamber slides at the seeding density of 30 000 cells per well for the 8 well chamber slide or 60 000 cells per well for the 4 well chamber slide.

Wells feature a hydrophobic border that defines their growth area.